Product Questions

What is Fully Enzymatic Synthesis™ (FES™)?

Fully Enzymatic Synthesis™ (FES) is our patented process for building high-quality, long oligonucleotides. Starting with an initiator DNA sequence, the first step is addition of a dNTP containing a 3’ phosphate blocking group using a proprietary, highly evolved TdT enzyme. Next, the phosphate blocking group is removed using a phosphatase enzyme to result in extension of the initiator by 1 nucleotide. This two-step cycle is then repeated until the desired length is reached. 

How do oligos produced using Fully Enzymatic Synthesis (FES) compare to oligos produced using chemical synthesis?

Our FES technology has an average cycle efficiency (CE) of 99.9%, compared to <99.6% for chemical synthesis, meaning that oligos produced using FES will have fewer sequence errors Because our cycle efficiency is higher than chemical synthesis, we can achieve higher purities at longer lengths. For example: a FES 200mer at 99.9% CE has an average purity of (0.999)^200 = 81.8%, while the analogous 200mer prepared by chemical synthesis at 99.6% CE only has (0.996)^200 = 0.448, or 44.8%.   

Do you offer PAGE or HPLC purification?

No, we do not offer purification other than standard desalting. Because of the high intrinsic accuracy of our process, additional purification is unnecessary to achieve high purity with our process 

How are oligos provided?

Our standard format is dried in tubes. However, you can request the oligos in other mediums such as resuspended in TE buffer or nuclease-free water, and in 96-well plates. 

How do I place an order?

To place an order, please fill out our form and provide it to your sales rep. We are working on an online ordering portal and will have some exciting news soon.

Do you screen the sequences for biosecurity?

Yes, every sequence is screened per the International Gene Synthesis Consortium (IGSC)’s Harmonized Screening Protocol. This protocol compares the DNA sequence to the Regulated Pathogen Database to identify any orders containing potentially dangerous sequences from viruses and toxins. Additionally, the protocol involves screening of customers to ensure that any regulated sequences identified are only provided to authorized laboratories. More information about IGSC can be found here.

Do you provide modifications?

Currently, we offer 3’P and 5’P modifications, with more coming soon. Please inquire with your sales rep for specific requests

When will my oligos be shipped?

Once you receive confirmation of your order, the typical turnaround time is less than 10 days for 200mers. We are working hard to decrease turnaround time and will continue to provide updates as available. 

How do you QC your oligos?

We use Liquid chromatography-mass spectrometry (LCMS) to verify oligos are the correct mass, and this data is included on the Certificate of Analysis (C of A). We also routinely perform Next Generation Sequencing (NGS) and Capillary Gel Electrophoresis (CGE) to check sequence quality.  

Product Usage

What will I get with my order?

Your oligo shipment will be accompanied by a Certificate of Analysis (C of A) sheet that includes LCMS results, oligo properties and total yield. 

How should I resuspend my oligo? 

Always centrifuge tubes prior to opening to ensure material is at the bottom of the tube. Resuspend in TE buffer or nuclease-free water to desired molar concentration. For example, a 5 uM solution can be prepared by reconstituting 50 pmol in 10 uL. 

How should I store my oligos?

Both dried and resuspended oligos should be stored in a freezer at -20°C. 

Transform Your Research With Fully Enzymatic Synthesis

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