Enabling CRISPR Knock-Ins

Explore how Molecular Assemblies Fully Enzymatic Synthesis (FES) enables high-efficiency Homology-Directed Repair (HDR) for CRISPR Gene Editing.

Molecular Assemblies partnered with Novohelix in their engineering of human pluripotent stem cells in which NovoHelix achieved a breakthrough in knock-in efficiency using MAI’s high purity ssODN HDR donors. We are excited to go into depth on their results in our latest white paper featuring:

Precision Transgene Integration

Utilization of Recombinase-Mediated Cassette Exchange high precision landing pad knock-ins to enable large transgene insertions.

Enabling Homology-Directed Repair

Challenges and strategies for overcoming the dominance of the Non-Homologous End Joining (NHEJ) pathway and increase HDR efficiency.